Rhode Island and Miriam Hospitals

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

Collins Cheruiyot¹, Zemplen Pataki¹, Robert Williams¹, Bharat Ramratnam^2,3, Ming Li³

¹Brown University, ²COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, ³Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.